This is a rather interesting climber from the tropics with long, slender stems twelve feet or more long clad with most attractive, five-lobed, somewhat vine-like. It is a tropical and subtropical vine of the family Cucurbitaceae, widely grown in Asia, Africa, and the Caribbean for its edible fruit, which is extremely bitter. Antileukemic Potential of Momordica charantia Seed Extracts on Human Momordica charantia (bitter gourd) has been used in the traditional.


Author: Nels Hansen III
Country: Libya
Language: English
Genre: Education
Published: 19 November 2016
Pages: 460
PDF File Size: 19.72 Mb
ePub File Size: 13.24 Mb
ISBN: 876-1-75624-208-6
Downloads: 49643
Price: Free
Uploader: Nels Hansen III


Taken together these results indicate that fractionated extracts of M. Introduction Acute myeloid leukemia AML is a complex disease, characterized by abnormal differentiation and momordica charantia seeds cellular proliferation that develops due to the accumulation of genetic and epigenetic alterations.

Current treatments including chemotherapeutic drugs, radiation, and stem cell transplantation are associated with incomplete remission and side effects [ 1 ].


Therefore, there is a need to discover novel anticancer drugs that are effective and have minimal side-effects associated with the treatment. In traditional system of medicine and folklores, plant-based formulations with potential anticancer properties have been described [ 23 ].

Investigating dietary compounds with potential chemopreventive and anticancer activity has provided important leads for the development of clinically relevant anticancer drugs [ 34 ] However, systematic scientific studies have been lacking to understand the mechanism s of their anticancer activity.

In the present study, we have focused our attention momordica charantia seeds a tropical plant, Momordica charantia L, belonging to Cucurbitaceae family that has been used in the traditional health care world over and the momordica charantia seeds use of this medicinal plant is well documented [ 56 ].

Evidence-Based Complementary and Alternative Medicine

Antigrowth properties of fractionated M. Subsequently, a number of growth inhibitors have been isolated from M. While antiproliferative and antitumor activity of M.

We have used human myeloid leukemic HL60 cells as an in vitro system to test the differentiation inducing potential of M.

HL60 cells have momordica charantia seeds used for screening of compounds for their antileukemic potential and to elucidate the molecular mechanisms involved in the differentiation process.

Different compounds have been reported to induce HL60 cells towards granulocytic, macrophagic, and momordica charantia seeds lineage [ 18 — 21 ]. In the present study, we report for the first time that the fractionated M.

Material and Methods 2. The chemicals required for cell culture were procured from Biological Industries, Israel. Other chemicals were from standard commercial suppliers.

Extraction and Partial Purification of M. All the fractions were evaporated and resuspended in Milli-Q water prior to use. Momordica charantia seeds concentrations of M. The blank sample contained medium only.

Momordica charantia - Wikipedia

After 72 h of incubation, the cells were centrifuged at rpm for 5 momordica charantia seeds and the supernatant culture medium carefully aspirated. The plate was incubated at momordica charantia seeds temperature with shaking for 10 min to solubilize formazan crystals.

The absorbance at nm was measured in a microplate reader Tecan, Switzerland. The amount of color produced is directly proportional to the number of viable cells.

Antileukemic Potential of Momordica charantia Seed Extracts on Human Myeloid Leukemic HL60 Cells

Cell viability was calculated using MTT absorbance of the control and treated cells: Viable cell counts were determined on each day post treatment using trypan blue dye exclusion assay using a light microscope Leica, Germany.

Momordica charantia seeds cells in triplicates were scored under a light microscope Leica, Germany for momordica charantia seeds test sample.


The data corresponding to the number of total NBT positive cells out of the total cells counted for different experiments are momordica charantia seeds in Table 1 in supplementary material available online at doi: